• The week in vitro culture

  2018-11-08

  

  The 15week in vitro culture protocol used in this study provided a very challenging culture system to examine long term potential of the cell sources. When grown in a large 6mm×9mm PCL scaffold, the AFS purchase Nocodazole had great potential but the cells required an extensive induction time prior

• How ABR participates in the control of mitotic

  2018-11-08

  

  How ABR participates in the control of mitotic fidelity remains an open question. Taking into consideration that ABR action is correlated with a cellular adhesive state, the present study suggests an unrecognized link between cell-cell contact and mitotic fidelity. In general, most types of non-tran

• Importantly using CXCL CXCR interactions as a paradigm for

  2018-11-08

  

  Importantly using CXCL12/CXCR4 interactions as a paradigm for other factors and receptors, we demonstrated that CXCL12 is required for chemotaxis, but not chemokinesis of human UCB CD133+ HSPCs in the absence of niche elements, and that the CXCR4 antagonist, AMD3100, inhibits chemokine sensing but n

• Because Foxd regulates stem cell properties in

  2018-11-08

  

  Because Foxd3 regulates stem cell properties in multiple lineages (Liu and Labosky, 2008; Hanna et al., 2002; Mundell et al., 2012; Mundell and Labosky, 2011; Teng et al., 2008; Tompers et al., 2005), Foxd3 target genes must regulate self-renewal, pluripotency, and/or survival of stem cells. Current

• br Materials and methods br Results br

  2018-11-08

  

  Materials and methods Results Discussion riPSCs are typically generated and cultured on MEF feeders (Liu et al., 2008). Feeders are sufficient to support rhesus iPSC long-term maintenance. However, this approach is too labor intensive for large-scale culture. Optimization of this culture sy

• ALS is a fatal neurodegenerative disorder

  2018-11-08

  

  ALS is a fatal neurodegenerative disorder characterized by a selective and widespread degeneration of lower and upper motor neurons (Gordon, 2013; Rowland and Shneider, 2001). The main symptomatic features of ALS are muscular atrophy, motor paralysis and difficulties in speaking, swallowing, chewing

• Immunocytochemistry Cells were fixed at room

  2018-11-08

  

  Immunocytochemistry. Cells were fixed at room temperature with 4% paraformaldehyde for 10min. Non-specific proteins were blocked by ultra V block (Thermo). The cells were then treated with primary The impact of overnight at 4°C. Primary antibodies were against TRA-1-60 (1:500, MA1-023, Thermo Fishe

• br Resource table Resource details Following their

  2018-11-08

  

  Resource table: Resource details Following their mechanical isolation from day 7 blastocysts, the inner cell masses (ICM) were seeded individually onto the feeder layers. After 2days of incubation, most of the ICMs were found attached to the feeder cells. The cultures were allowed to grow for 8–

• However the relative contribution of factors provided

  2018-11-08

  

  However, the relative contribution of factors provided by the different niche transferase including the precursor cells themselves, endothelial cells, pericytes, neurons and astrocytes to this concert has not yet been systematically explored for the hippocampus. There are no widely usable in vitro s

• Here we demonstrate that the methylation patterns of the H

  2018-11-08

  

  Here, we demonstrate that the methylation patterns of the H19/IGF2 and SNRPN DMRs were stable from the early stages of derivation to late passages of monkey ESCs, and following in vitro differentiation. We also showed that the H19/IGF2 DMR methylation pattern was correlated with the capacity of earl

• Sca expression has earlier been

  2018-11-08

  

  Sca-1 expression has earlier been shown to mark p53 apoptosis with poor CFE (Jensen et al., 2008; Jensen et al., 2009). We can now for the first time show that a PletA6Sca is a highly proliferative subpopulation of Plet-1pos cells that displays almost as efficient colony forming efficiency as A6, b

• Recently work has been focused on the adipose tissue derived

  2018-11-08

  

  Recently, work has been focused on the adipose tissue-derived stromal cell (ASCs) population, since retrieval is straight forward and non-harmful coupled to low-risk surgical procedures. However, biological properties of ASCs have only been assessed by indiscriminate isolation from mostly white fat

• Subsequent differentiation of stem cell derived MK

  2018-11-08

  

  Subsequent differentiation of stem cell-derived MK progenitors into mature MKs and ultimately platelets has been notoriously difficult. Coculture with OP9 or C3H10T1/2 stroma dopamine receptor antagonist has been found to greatly facilitate the process (Lu et al., 2011; Takayama et al., 2008), but

• br Results br Discussion In this

  2018-11-08

  

  Results Discussion In this study, we integrated transcriptome and proteome data and characterized the inventory of HSPCs and their immediate myeloid committed progeny (LS−K) (Figure S6). With almost 20,000 identified expressed genes including more than 3,000 significantly regulated ones, these

• Whereas most studies aiming at reprogramming brain resident

  2018-11-08

  

  Whereas most studies aiming at reprogramming brain-resident glia into neurons focused on astroglia, NG2 glia represent an interesting alternative cellular source given their abundance and life-long capacity for proliferation (Dimou and Götz, 2014). Here, we found that ∼60% of the induced DCX+ neuron

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