• Our survey of the effects of radiation

  2018-10-22

  

  Our survey of the effects of radiation, hypoxia, and gemcitabine on the SP shows that there may not be a consistent effect on this sub-population. This suggests that the SP alone may not be the best method to isolate pancreatic cancer stem cells. Other studies have found that CD24 and ALDH are enric

• The donor to donor proteomic variability documented in this

  2018-10-22

  

  The donor-to-donor proteomic variability documented in this paper can help to explain previous reports that show functional discordance between cell populations from different donors (Phinney, 2007, 2012). As per our data, only 13% of the total proteins were found universally expressed by all cell l

• Although our work with BRCA mutant iPSCs

  2018-10-22

  

  Although our work with BRCA1 mutant iPSCs led to our investigation of PKC-theta upregulation in human breast tumors, future studies will be necessary to validate whether PKC-theta can be used as a biomarker or therapeutic target in BRCA1 mutant tumors. Because the frequency of BRCA1 mutations in pat

• The ability of diflunisal and flufenamic acid known TTR

  2018-10-22

  

  The ability of diflunisal and flufenamic acid, known TTR tetramer stabilizers, to prevent iPSC-derived target cell death in the presence of ATTRL55P hs validates this system as a potential platform in which to test novel therapeutic agents. Different approaches to therapeutically target the cause an

• In pluripotent cells phosphorylation has a central role in

  2018-10-22

  

  In pluripotent cells, phosphorylation has a central role in directing cell identity by relaying growth-factor signaling through key pathways (i.e., fibroblast growth factor [FGF] and transforming growth factor β [TGF-β]) (Chen et al., 2011; Singh et al., 2012a; Vallier et al., 2005; Yu et al., 2011)

• br Results br Discussion Culturing hFK in mNPEM instantly

  2018-10-22

  

  Results Discussion Culturing hFK in mNPEM instantly showed morphological heterogeneity, including specific undifferentiated stem cell niches and differentiating epithelial cells, which could be further exploited to show an EpCAM expression gradient (EpCAM−, EpCAMdim, EpCAMbright) within the NC

• Main Text It is remarkable that only years

  2018-10-22

  

  Main Text It is remarkable that only 20 years after the first report of the growth of human embryonic stem order JWH 073 in vitro and just 10 years after discovering that somatic cells can be reprogrammed to pluripotency as induced pluripotent stem cells (iPSCs), the results of the first clinical t

• The functional in vitro data using

  2018-10-22

  

  The functional in vitro data using high-sensitive ELISA suggested that the 3D model-derived TH+ neurons secrete DA at a higher level than hESCs derived from the conventional 2D platform upon K+ induction. However, it is recommended that the ELISA data be validated via high performance liquid chromat

• apelin receptor To identify the label retaining

  2018-10-20

  

  To identify the label-retaining apelin receptor within the myocardium, BrdU was administrated and the intensity of the BrdU signal was examined after a prolonged chasing period (Urbanek et al., 2006). As expected, the number of bright BrdU-CSCs in the atria decreased rapidly after chasing while the

• We asked whether the physiological properties of

  2018-10-20

  

  We asked whether the physiological properties of neurons contained in our SFEBs were similar to what has been observed in other iPSC-derived neurons. Thus, we conducted whole-cell patch clamp recordings 30days after SFEBs had been placed on the MI (day 45 of differentiation) (Fig. 5A). We evoked act

• br Materials and methods The PQ embryos from

  2018-10-20

  

  Materials and methods The PQ embryos from in vitro fertilization were donated, and the fetal membranes were collected in accord with the INPER Ethics Committee (212250-21071) as well as the Guidelines for the Conduct of hESC Research (ISSCR, 2006). The hAEC were isolated and cultured from human

• Following fluorescence activated cell sorting FACS for dToma

  2018-10-20

  

  Following fluorescence activated cell sorting (FACS) for dTomato-reporter expression, HSPCs were maintained for ten days in liquid culture using StemSpan medium (StemCell Technologies, Grenoble, France) supplemented with 1% penicillin/streptomycin, 1% l-glutamine (Biochrom AG, Berlin, Germany) and t

• br Methods br Results br Discussion

  2018-10-20

  

  Methods Results Discussion Our method for osteoprogenitor cell culture is similar to protocols for mice (Meirelles and Nardi, 2003; Soleimani and Nadri, 2009; Nardi and Camassola, 2011; Abdelmagid et al., 2014; Fig. 1). Marrow and cortex-derived cell populations of bats and mice shared simi

• Further cultivation of iSABs for weeks

  2018-10-20

  

  Further cultivation of iSABs for 3 weeks on gelatin-coated dishes led to attached, highly synchronized cell layers beating at >350 bpm (Movie S4). The Cyclosporin A exhibited the typical shape of nodal cells (i.e., spindle and spider-shaped cells; Figure 3C; Movie S5). To address the electrophysio

• tyrosol Although the present study focuses predominantly on

  2018-10-20

  

  Although the present study focuses predominantly on keratinocytes, it has been known for many years that fibroblasts play an important role in wound healing and in remodeling the extracellular matrix. Thus, an ideal bioengineered graft will always need to incorporate fibroblasts as well as keratinoc

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