Following fluorescence activated cell sorting FACS for dToma

2018-10-20

Following fluorescence activated cell sorting (FACS) for dTomato-reporter expression, HSPCs were maintained for ten days in liquid culture using StemSpan medium (StemCell Technologies, Grenoble, France) supplemented with 1% penicillin/streptomycin, 1% l-glutamine (Biochrom AG, Berlin, Germany) and t

br Methods br Results br Discussion

2018-10-20

Methods Results Discussion Our method for osteoprogenitor cell culture is similar to protocols for mice (Meirelles and Nardi, 2003; Soleimani and Nadri, 2009; Nardi and Camassola, 2011; Abdelmagid et al., 2014; Fig. 1). Marrow and cortex-derived cell populations of bats and mice shared simi

Further cultivation of iSABs for weeks

2018-10-20

Further cultivation of iSABs for 3 weeks on gelatin-coated dishes led to attached, highly synchronized cell layers beating at >350 bpm (Movie S4). The Cyclosporin A exhibited the typical shape of nodal cells (i.e., spindle and spider-shaped cells; Figure 3C; Movie S5). To address the electrophysio

tyrosol Although the present study focuses predominantly on

2018-10-20

Although the present study focuses predominantly on keratinocytes, it has been known for many years that fibroblasts play an important role in wound healing and in remodeling the extracellular matrix. Thus, an ideal bioengineered graft will always need to incorporate fibroblasts as well as keratinoc

To summarize this study demonstrates that FMR epigenetic gen

2018-10-20

To summarize, this study demonstrates that FMR1 epigenetic gene silencing can take place in undifferentiated FXS prolyl hydroxylase inhibitor and underscores the importance of examining multiple cell lines when investigating epigenetically regulated disorders. Experimental Procedures Author C

Given the emerging role of

2018-10-20

Given the (1) emerging role of IL-3 in early primitive as well as definitive hematopoietic specification (Donahue et al., 1988; Robin et al., 2006; Yang et al., 1986), (2) the fundamental importance of G-CSF and M-CSF in terminal granulocyte and monocyte/macrophage differentiation (Clark and Kamen,

br Resource table Resource details

2018-10-20

Resource table. Resource details Macaca fascicularis iPS RO 4929097 ATCi-MF1 were generated from adult skin-derived fibroblasts by transduction with non-integrative Sendai viruses coding for the reprogramming factors OCT3/4, KLF4, SOX2 and c-MYC (Ban et al. 2011; Zapata-Linares et al. 2016). Tra

We generated immortalized BMSC clones to perform detailed an

2018-10-20

We generated immortalized BMSC clones to perform detailed analysis of functional diversity in BMSC subtypes to levels not previously described. Similar immortalization techniques (e.g., hTERT combined with human papillomavirus E6/E7) have been used for the clonal analysis of BMSCs, which found OAC,

Previous reports have shown that inhibition of

2018-10-20

Previous reports have shown that inhibition of GSK3 by CHIR promotes mESC self-renewal through activation of Wnt/β-catenin signaling pathway (Martello et al., 2012). In the absence of Wnt ligand or CHIR, T cell factor 3 (TCF3) occupies the promoter regions of many pluripotency genes (e.g., Nanog, Es

However PSCs have major safety concerns such as teratoma

2018-10-20

However, PSCs have major safety concerns, such as teratoma formation due to possible contamination of the residual undifferentiated PSCs (Lee et al., 2013a). Teratoma is a unique characteristic of PSCs owing to their pluripotency and ability to undergo unlimited proliferation (Gruen and Grabel, 2006

Researchers are usually aware of the tissue that was

2018-10-20

Researchers are usually aware of the tissue that was initially used for isolation of MSCs, but there is evidence that accidental interchange of samples or contaminations with other GSK256066 can occur (Garcia et al., 2010; Torsvik et al., 2010). For established cell lines, some contaminations can b

The inhibition of differentiation has been considered to pla

2018-10-20

The inhibition of differentiation has been considered to play a role in many types of tumor development through maintenance of the proliferating progenitor cell state. Previous studies demonstrated that the knockdown of EWS-FLI1 in Ewing sarcoma cell lines results in osteogenic, adipogenic and chond

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2018-07-29